-
Major Cities along I-81 Interstate 81
-
South
- Tennessee
- Knoxville
- Morristown
- Greeneville
- Johnson City
- Kingsport
- Bristol
- Virginia
- Bristol
- Blacksburg
- Roanoke
- Winchester
- West Virginia
- Martinsburg
- Maryland
- Hagerstown
- Pennsylvania
- Chambersburg
- Carlisle
- Harrisburg
- Hazleton
- Wilkes-Barre
- Scranton
- New York
- Binghamton
- Cortland
- Syracuse
- Watertown
-
North