State Route 227 or SR-227 is a 15-mile (24 km) long route that serves as a connection between the DeKalb/Etowah county line south of Crossville with U.S. Highway 431 at Guntersville in Marshall County.

The southern terminus of SR-227 is located at the DeKalb/Etowah county line to the southwest of Hendrixville. From Hendrixville, the route travels in a northwesterly direction in route to Crossville. From Crossville, it travels in a northerly direction to Geraldine where it again resumes its northwesterly track.

Upon entering Marshall County, it takes a more westerly route before turning towards the south in route to its northern terminus at U.S. Highway 431 in downtown Guntersville.

The southern terminus of SR-227 until approximately 1985 occurred on Meighan Boulevard in Gadsden, Alabama which is also US 278/US 431. The routing ran northward passing near the Noccalula Falls Park then crossing Interstate 59 (exit 188), followed by an intersection with US 11 near Reece City and continuing onto Crossville, Alabama. The route between Meighan Boulevard in Gadsden and US 11 in Reece City is now designated as SR-211. The remainder of the former route between US 11 and the Etowah/DeKalb county line is now an Etowah county road until the SR-227 designation begins at the county line.