State Route 28 is a 97-mile (156 km) long state route in the western part of the state. The route’s western terminus is at the junction with State Route 17 at Emelle in northwestern Sumter County, and the eastern terminus of the route is at the junction with State Route 21 near Darlington in eastern Wilcox County.

From its beginning in Sumter County, SR-28 travels in a general southeastwardly trajectory through Livingston, the home of the University of West Alabama. After leaving Livingston, the route passes through the Black Belt region of Alabama, one of the state’s poorest regions. The only towns whose population exceeds 2,000 that SR-28 passes through are Livingston, Linden, and Camden. East of Camden, the route assumes a more eastwardly trajectory until its terminus approximately 13 miles (21 km) east of the town.