U.S. Route 395 (US 395) clips a corner of the U.S. state of Nevada near Lake Tahoe and serves the cities of Gardnerville, Minden, Carson City and Reno. All of US 395 north of Carson City is a freeway and is built up to Interstate standards. Part of the freeway section is also designated as Interstate 580.

U.S. 395 enters Nevada at Topaz Lake as a two lane highway and gradually winds its way to Carson Valley where it becomes the principal thoroughfare for the Minden-Gardnerville area. Upon entering the Carson Valley 395 becomes a 4 lane highway through Minden and Gardnerville. Upon leaving Minden the highway becomes a 4 lane divided highway and turns due north towards Carson City.

The highway currently serves as Carson Street, the main street of Carson City. However, a freeway bypass is currently under construction, with the northern half open to traffic. Temporarily US 395, and US 50, are routed along Fairview Drive to connect the freeway and Carson Street. This freeway is planned to become Interstate 580 but is currently signed as US 395.

Upon exiting Carson City, the highway continues north through the Washoe Valley. Midway through the valley the freeway portion temporarily ends. The highway also crosses Pleasant Valley before entering Reno. A new freeway portion through Pleasant Valley is also currently under construction. Upon entering Reno the freeway portion resumes as a 6 lane freeway and is designated the Martin Luther King Jr. freeway. The highway heads north to intersect Interstate 80 near downtown Reno. Shortly afterward, US 395 turns northwest to serve the north valleys of greater Reno area before returning to California at Bordertown.

The entire route through Nevada closely follows the eastern edge of the Sierra Nevada range. Several prominent peaks are visible from the highway, including Jobs Peak and Mount Rose.