Route 240 is a highway in the central part of Missouri. Its eastern terminus is at Interstate 70/U.S. Route 40 about two miles (3 km) west of Columbia; its western terminus is at U.S. Route 65 in Marshall. Spur Route 240 connects the road with Rocheport.

From 1922 until 1926, Route 240 from Rocheport to Fayette was Route 67. It was renumbered Route 3 in 1926; that route has since been extended north and truncated, leaving Route 240 behind. The Route from Fayette to Glasgow has since been Re-Routed along with the bridge replacement, heading South to MO-87, Then going toward the river. MO-5 then heads North to Keytesville. The old road is now Business Loop 240/Business Loop 5.

The highway was mentioned in the title of David R. Holsinger's four-movement piece "One Day In a Small Town...". The fourth movement is titled "Chasing the Band Bus on MO240".