North Carolina Highway 27 is a primary state highway in the state of North Carolina. The route traverses 198 miles (319 km) through southern and central North Carolina, about 100 miles (160 km) of it as a concurrency with North Carolina Highway 24.

NC 27 begins in Cleveland County near the unincorporated community of Toluca at a T-intersection with NC 10. From there it runs southeast to the city of Lincolnton. In Lincolnton, it serves as Main Street, and runs past the Lincoln County courthouse. From Lincolnton, it runs southeast again to Stanley where it follows Main Street. It enters Charlotte along Mount Holly Road, and follows several major thoroughfares through Charlotte, including Freedom Drive, Morehead Street, and Independence Boulevard.

On the east side of Charlotte, it begins its 100-mile (160 km) long concurrency with NC 24, approximately 1/2 of its total length. The two highways leave the city along Albemarle Road and remain joined until the unincorporated community of Johnsonville. Along this segment, they pass through the cities of Midland, Locust, Albemarle, Troy, Biscoe, and Carthage. They share further concurrencies with NC 109, NC 22, and US 15.

From the split with NC 24, the road runs northeast to Lillington where it follows Main Street, and leaves town as a concurrency with US 421. It passes Campbell University in Buies Creek before entering Johnston County and ending in Benson just short of I-95. NC 50 continues east of NC 27's terminus at US 301.

NC 27 was one of North Carolina's original 1922 state highways. Its original routing connected Charlotte to Lincolnton. It used several streets through Charlotte, but followed roughly the same route. The road was extended several times:

NC 27 has had a tumultuous history through Charlotte. It has always served as a major east–west route through the city, but it has been rerouted numerous times on different city streets as traffic patterns changed. All of the following roads have carried NC 27 at one time or another:

NC 27, west of Lincolnton.