Virginia State Route 90 (SR 90) is a primary state highway in the U.S. state of Virginia. Known as Main Street, the state highway runs 1.73 miles (2.78 km) from SR 616 and SR 749 north to Interstate 81 (I-81) within Rural Retreat in western Wythe County. SR 90 connects Rural Retreat with I-81 and U.S. Route 11 (US 11).

SR 90's southern terminus is at the center of the town of Rural Retreat at the intersection of Main Street and Baumgardner Avenue. Main Street continues south as SR 749 and Baumgardner Avenue is SR 616 in both directions. The state highway heads north and has an at-grade crossing of Norfolk Southern Railway's Pulaski District. SR 90 leaves the town limits and intersects US 11 (Lee Highway) at Staley Crossroads. The state highway continues to its northern terminus at the southbound ramps of its diamond interchange with I-81. The roadway continues north as SR 680 (Blacklick Road).

The entire route is in Rural Retreat, Wythe County.