Interstate 395 (abbreviated I-395) is a 67-mile-long north–south Interstate Highway that begins at Interstate 95 in East Lyme, Connecticut and ends at Interstate 90 in Auburn, Massachusetts, where it becomes Interstate 290. The original designation for the freeway was Route 52. Route 52 was slated to become an extension of Interstate 290 before it received the I-395 designation in 1983.

From its southern terminus to its intersection at Exit 90 in Plainfield, Connecticut with State Road 695, I-395 is also part of the Connecticut Turnpike.

From East Lyme to Plainfield, Connecticut, I-395 is part of the Connecticut Turnpike (the Gov. John Davis Lodge Turnpike). From Plainfield to Thompson, Connecticut, I-395 is named The American Ex-Prisoner of War Memorial Highway.

In Connecticut, there are three Service Plazas that provide 24-hour gas stations and convenience stores. The renovated Plainfield plazas have Subway, Dunkin' Donuts, and Fast Freddie's convenience stores. The service area in Montville does not have restaurants but does have phones and rest rooms.

A State Police station is located on the northbound side of the highway in Montville in what used to be another Service Plaza.