Wyoming Highway 271 is an east–west Wyoming State Road known as Twenty Mile Road and is located in west-central Niobrara County.

Wyoming Highway 271 travels from Wyoming Highway 270 at Lance Creek west 3.19 miles to West Lance Creek where it ends and Niobrara CR 23 takes over. Milepost zero is at its eastern junction with Wyoming Highway 270. WYO 271 also provides access to the local airport.

The entire route is in Niobrara County.